Journal: Oncogene

Article Title: CREPT facilitates colorectal cancer growth through inducing Wnt/β-catenin pathway by enhancing p300-mediated β-catenin acetylation

doi: 10.1038/s41388-018-0161-z

Figure Lengend Snippet: CREPT enhanced CRC metastasis in vitro and in vivo. Ectopic expression of CREPT-promoted cell invasion a and migration b in HCT116 cells. Knockdown of CREPT suppressed cell invasion c and migration d in SW480 cells. e Ectopic expression of CREPT accelerated lung metastasis of HCT116 cells injected via tail vein. Pictures on the left showed representative lung morphology. HE staining showed representative lungs section. Histograph showed statistic result of metastasis nodules in nude mice lung tissues. n = 5, * P < 0.05, ** P < 0.01

Article Snippet: Human CRC cell lines (CaCO2, Colo205, DLD-1, HCT116, HT29, LoVo, SW1116, SW480, and SW620) and one immortalized colorectal epithelial cell line (NCM460) were from the American Type Culture Collection (Manassas, VA) and were cultured as described in previous study [ ].

Techniques: In Vitro, In Vivo, Expressing, Migration, Knockdown, Injection, Staining

Journal: Oncogene

Article Title: CREPT facilitates colorectal cancer growth through inducing Wnt/β-catenin pathway by enhancing p300-mediated β-catenin acetylation

doi: 10.1038/s41388-018-0161-z

Figure Lengend Snippet: CREPT regulated Wnt/β-catenin signaling pathway in CRC cells. a KEGG analysis based on RNA-sequencing data of HCT116 cells stably overexpressing CREPT or control cells. b Ectopic expression of CREPT in DLD-1 cell line enhanced TopFlash luciferase reporter activity but not FopFlash reporter ( b1 ), while depletion of CREPT reduced dual-luciferase reporter assays in SW480 cells ( b2 ). TopFlash, a classic Wnt-response luciferase reporter containing 3xTCF4 binding site. FopFlash, a negative reporter of which TCF4 binding site is mutant. c The mRNA expression of Wnt downstream targets CCND1 , c-MYC , and AXIN2 were enhanced in the presence of CREPT in DLD-1 cells and HCT116 cells. d The mRNA expression of CCND1 , c-MYC , and AXIN2 was diminished under CREPT knockdown in HT29 and SW480 cells. e The protein expression of β-catenin, c-MYC, CYCLIN D1, and AXIN2 was increased upon ectopic expression of CREPT in DLD1, HCT116 ( e1 ), HCEC 1CT, and 2CT cells ( e2 ), while decreased in CREPT knockdown HT29 and SW480 cells ( e3 ).

Article Snippet: Human CRC cell lines (CaCO2, Colo205, DLD-1, HCT116, HT29, LoVo, SW1116, SW480, and SW620) and one immortalized colorectal epithelial cell line (NCM460) were from the American Type Culture Collection (Manassas, VA) and were cultured as described in previous study [ ].

Techniques: RNA Sequencing, Stable Transfection, Control, Expressing, Luciferase, Activity Assay, Binding Assay, Mutagenesis, Knockdown